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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#_3 http://www.w3.org/ns/prov#value Androgens inhibit the growth of breast cancer cells, but the mechanism of androgen-induced growth inhibition has not yet been elucidated, and few androgen-responsive genes have been identified. We, therefore, used differential display PCR to identify novel androgen-responsive genes in ZR-75-1 human breast cancer cells. The human UDP-glucose dehydrogenase gene (UDPGDH), which was not known to be androgen regulated, was detected and cloned by complementary DNA library screening. The UDPGDH open reading frame codes for a protein of 494 amino acids that migrates at an apparent molecular mass of approximately 54 kDa. Northern blot analysis revealed the existence of two messenger RNA species of approximately 3.5 and 2.7 kb in all of the human breast cancer cell lines examined. The major UDPGDH transcript was induced rapidly (within 6 h) by dihydrotestosterone in ZR-75-1 cells, and a maximal 13-fold induction was observed after 24 h of treatment. http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10499502 http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10499502 http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#_3 http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A#pubinfo http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A http://purl.org/dc/terms/created 2014-07-03T14:29:48.549+02:00 http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA-psHCCERk5bzLAO37u59aRTOtZ9x2qOSPMD-5ZZms3A http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234