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Incubation of serum-deprived cells on fibronectin and/or ITGB1-coated dishes for 15 minutes resulted in integrin-mediated strong phosphorylation of STAT5A, showing that this event was integrin specific. Tyrosine phosphorylation of STAT5A occurred within 15 minutes of adhesion to matrix proteins and completely disappeared within 30 minutes, indicating an early and transient event in integrin signaling.
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