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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#_5 http://www.w3.org/ns/prov#value Figure 5B shows that addition of either of the two anti-NFYA antibodies to the binding reactions supershifted a prominent complex (lanes 3 and 4) and the anti-NFYB antibody diminished binding significantly (lane 5). To test the functional effect of NFY binding to the +60/+64 region, we introduced point mutations abolishing the binding of NFY to its site (see Fig 5A and Fig 5B; lane 14) and incorporated them into a luciferase plasmid containing base pairs -391/+175 from the human CD34 promoter (constructs diagramed in Fig 5C). As shown in Fig 5D, mutating the NFY site (Y-M+ construct) resulted in a 5-fold decrease in luciferase activity in transiently transfected KG1a cells and a 4-fold decrease in RPMI-8402 cells, as compared with the wild-type construct (Y+M+ construct). http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10572091 http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10572091 http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#_5 http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE#pubinfo http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE http://purl.org/dc/terms/created 2014-07-03T14:29:49.325+02:00 http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA3qeLHeqqYAcGR6c3KRYgUnRP3vXrMVF6jEWR5CdX6bE http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234