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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#_3 http://www.w3.org/ns/prov#value Spontaneously immortalized wild type mouse fibroblasts were mock-irradiated or irradiated with x-rays and harvested after 30 min, and H2AX phosphorylation was analyzed by Western blotting of SDS extracts with anti-?-H2AX antibody. We observed significant phosphorylation of histone H2AX in response to ionizing radiation (Fig.1 b). The observed phosphorylation was specific to serine 139. Significant phosphorylation of H2AX was also observed after treatment of cells with the DSB-inducing agents neocarzinostatin, bleomycin, and etoposide. http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11571274 http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11571274 http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#_3 http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0#pubinfo http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0 http://purl.org/dc/terms/created 2014-07-03T14:29:58.282+02:00 http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA3wAXM5Z8utxq9uSzOP42o1rBOIh_A0XA74ZiE_0NFa0 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234