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[Fresh whole blood from 20 patients with allergic rhinitis and 19 healthy donors was used to investigate the frequencies of CD4(+)IFN-?(+) T cells (effector T helper-1), CD4(+)IL-4(+) T cells (effector T helper-2) and CD4(+)IL-17a(+) T cells (effector T helper-17) by flow cytometry after stimulation for 4-6 h. Simultaneously, CD4(+) T lymphocytes were isolated from peripheral blood mononuclear cells and then the frequencies of regulatory T cells and IL-17a-producing regulatory T cells in the allergic rhinitis group were compared with healthy controls after T-cell antigen receptor stimulation for 48 h. The suppressive capacity of CD4(+)CD25(high)CD127(low) regulatory T cells that were stimulated by Dermatophagoides pteronyssinus in both groups was isolated and assessed.]. Sentence from MEDLINE/PubMed, a database of the U.S. National Library of Medicine.
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