http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#Head http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#provenance http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#pubinfo http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:88439 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://amigo.geneontology.org/amigo/term/GO:0018108 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_1 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.w3.org/2000/01/rdf-schema#label p(MGI:Cntf) -> bp(GO:"peptidyl-tyrosine phosphorylation") http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_3 http://www.w3.org/ns/prov#value We have identified by Scatchard analysis both high (124 pM, 14.4 x106 sites/micrograms protein, 7600 sites/cell) and low (1.6 nM, 7.7x106 sites/micrograms protein, 4100 sites/cell) affinity receptors for [125I]-rat ciliary neurotrophic factor (rCNTF) on astrocytes. Ligand competition studies showed that the binding of [125I]-rCNTF was effectively competed by rCNTF and human CNTF, but not by hLIF, mIL-6 or mIL-1B. Three proteins specifically crossed-linked to [125I]-rCNTF, with the molecular weights of 190, 100, and 43 kDa, were immunoprecipitated by anti-rCNTF antibodies. Anti-LIFR or anti-gp130 antibodies immunoprecipitated the 100 and the 190 kDa proteins. CNTF induced the tyrosine phosphorylation of LIFR and gp130, as well as of proteins with the molecular weights of 88/91 and 42 kDa. The phosphorylation of the 88/91 kDa protein(s) was inhibited by pretreating the cells with staurosporine, 12-myristate 13-acetate phorbol (PMA), W7, chlorpromazine, or the intracellular Ca+2 chelator BAPTA/AM. In contrast, CNTF and PMA acted synergistically to induce the phosphorylation of two proteins with the molecular weights of 42 and 44 kDa. At later time points following CNTF treatment, c-fos messenger RNA and protein levels were increased. Collectively, these data indicate that hippocampal astrocytes express high-affinity, biologically functional receptor complexes for CNTF. http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10082809 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10082809 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#_3 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU#pubinfo http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://purl.org/dc/terms/created 2014-07-03T14:29:46.239+02:00 http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA9Y0d1FPF93Y25z23ZPLkXT0dgGzsj8fJNvcrzG-1azU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234