@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix this: <http://purl.org/np/RAAKLPi0aHaXo9HkBkkJaYMCBnz4-ANvWiV-nnDbC05Nk> .
@prefix sub: <http://purl.org/np/RAAKLPi0aHaXo9HkBkkJaYMCBnz4-ANvWiV-nnDbC05Nk#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_29362 rdfs:label "ethylene carbonate" .
  
  obo:CHEBI_35225 rdfs:label "imaging buffer" .
  
  <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> dce:description
      "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics.";
    sp:hasExperimentalInput obo:CHEBI_29362;
    bs:bioSampleUsed obo:CHEBI_35225;
    bs:labEquipmentUsed obo:CHEBI_35225;
    bs:reagentUsed obo:CHEBI_35225 .
}

sub:provenance {
  sub:assertion prov:generatedAtTime "2020-11-14T18:05:21.158199"^^xsd:dateTime;
    prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw, authority:FrhgjABcEeu5B4dnXgvb_A,
      authority:d5JrdABbEeuatj9X3vvoXw .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
    npx:hasSignature "SWmpxR9xIzSU30M5K3B5BRDabexhqu+Owx8yPz9mW7Ij7xmUF5S1kVs/6DMcZgrYMYGgbFJ6O1iEQIeBzKin4Quo4euDaZO51+kjl8y/pTChtXuaFjcDsMfCpZ7ZlT3dUGak8jVLr4u4Zd4HmfRWNj1K5/SYGcgqN7Mv30h+4io=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2020-11-14T18:05:21.158199"^^xsd:dateTime;
    prov:wasAttributedTo <acct:miguel.ruano@hypothes.is> .
}