@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAB6KCX8--1IiEIeic7OAcgBC0QBXBuw6eTNf8Oht-bJQ> .
@prefix sub: <http://purl.org/np/RAB6KCX8--1IiEIeic7OAcgBC0QBXBuw6eTNf8Oht-bJQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_30879 rdfs:label "alcohol" .
  
  sub:_1 rdfs:label "mosquitoes" .
  
  sub:_2 rdfs:label "mosquitoes" .
  
  sub:_3 rdfs:label "phosphate buffer saline(PBS)" .
  
  sub:_4 rdfs:label "sterile distilled water" .
  
  sub:step dce:description "Wash preserved mosquitoes in sterile distilled water or phosphate buffer saline(PBS) to remove excess alcohol. Fresh mosquitoes can be ground directly.";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:reagentUsed obo:CHEBI_30879, sub:_3, sub:_4 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-24T15:58:13.814624"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:954NunbeEeuT5V-mJXYEzw, authority:C52DBHbfEeuzW9fTMeff6g,
      authority:KAaxlnbfEeujqz-pwOV-nw, authority:RxhElnbfEeujr5dJrL1iNw, authority:Z4vxgnbfEeujrb-STXzizA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "CIoLYZfpap7qq1stiVMGp2tyqj4LR/RXIV3dYEwRsGqCPnnQtLhzqxIWmugoVCtXLcCIfJHctpJhPuew+UY+JuamyFg1m32u4uXxxNMA3jTwno20ka3jpyReE9CInim/2ZOA5fOxkMaM8q+xy/sqKx/bzKuk+KRxW0wUwCclwWk=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-24T15:58:13.814624"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}