@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> .
@prefix sub: <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_17824 rdfs:label "isopropanol" .
  
  sub:_1 rdfs:label "clear supernatant" .
  
  sub:_2 rdfs:label "clear supernatant" .
  
  sub:step dce:description "Transfer the very clear supernatant to a fresh tube , add two fold volume cold  isopropanol and keep it for 1 hour at -20º celcius";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed obo:CHEBI_17824 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:40:34.285030"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1AI99nJPEeuV0jNgsqleMQ, authority:3_u-coG2EeuImTcXZHRdwA,
      authority:FPj_NHJQEeusgDdsyq658w, authority:Lh_nmHJQEeuT8WfgFIk0fw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "U1qf30RAXfxn1/xwTczDFhzx1wBXAs8h4UBAJ4OiTXKiqKhZXKRqbrholoeYD6OmZ8aEf4Z8QLIMPo2jO/5HtS6Sb81GV/WhsNeoMxYp7eJsEDJiVIeR2Qz8dq3xJnwLVwt6IK6cj57brAhPPZCYiv5YUR8oJ4DkZKpv4zV+YVk=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:40:34.285030"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}