@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAE99H9MwYVHT12e7hbsUsBBLdAuQv0jr7JInE5tFiPs4> .
@prefix sub: <http://purl.org/np/RAE99H9MwYVHT12e7hbsUsBBLdAuQv0jr7JInE5tFiPs4#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_35255 rdfs:label "chloroform" .
  
  sub:_1 rdfs:label "Cell lysates " .
  
  sub:_2 rdfs:label "Cell lysates " .
  
  sub:_3 rdfs:label "eppendorf tubes" .
  
  sub:step dce:description "Cell lysates were transfer into eppendorf tubes and one-fifth (volume/volume) of chloroform was added to each tube.";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed sub:_3;
    bs:reagentUsed obo:CHEBI_35255 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-18T09:48:11.811790"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:7_X7wnE-Eeuw3kNBSTg_-A, authority:CbGn5nH4Eeuv6guNO1r8uA,
      authority:EbCxOnE_Eeur9ytFzTfK7w, authority:Om7khHE_EeunexslNNPfLA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "CdzFbuup4Q9hPPF0ngwnWt8W8OAQektRSzEYZPMPrpwL9KAp93ZkX4J8jd+OFEJQSvTutWN0G4q8ySfad5c1jseLuC9iy/fSWQZN4qMDnub58DAJ13poJpuv+fklNnRdzng48n7Jjfg4NhtmmsmyWDn7xH8mwor2t47UyE+ttwA=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-18T09:48:11.811790"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}