@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAFnW_6_oJ0XkkGp14SKHJ3-vMqYOm5mQS0x3-IsC0tXA> .
@prefix sub: <http://purl.org/np/RAFnW_6_oJ0XkkGp14SKHJ3-vMqYOm5mQS0x3-IsC0tXA#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:UBERON_0000178 rdfs:label "blood" .
  
  sub:_1 rdfs:label "auto pipette" .
  
  sub:_2 rdfs:label "Ficoll Histopaque " .
  
  sub:step dce:description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers.";
    a pplan:Step;
    bs:bioSampleUsed obo:UBERON_0000178;
    bs:labEquipmentUsed sub:_1;
    bs:reagentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-14T04:57:00.692150"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5-e5jlbqEeuzmHdPRwV2qg, authority:_zUYcFbqEeu_oBOB4SvIBA,
      authority:efY5qlbqEeuPWPMIV2Luiw, authority:yi5knFbqEeubIXflMrfRXg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "feKpHojqvxkYum9m4+EkBUtSJ122l9dwzQIY/bb2CCt3E/eqwa/POGDsUd9q9+zM4Mm1Du3O8Ii6IGg3wwvJj0BFLb/HiU1eqRAuwIkNlSe86yJMLE7oPpAdompzKpGY8/nAe/YlVcJK0D1OtPCqUdMzEgZ0ZCGHkNoLMEE4Oyo=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-14T04:57:00.692150"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}