@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAGLvb5xIqJNxIDci1Kkmct7CQlY_7oum6XSpmhUI7Oyc> .
@prefix sub: <http://purl.org/np/RAGLvb5xIqJNxIDci1Kkmct7CQlY_7oum6XSpmhUI7Oyc#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   obo:OBI_0000136 rdfs:label "incubator" .
  obo:OBI_0400155 rdfs:label "water bath" .
  sub:step dce:description "The falcon tube was kept into the 65°C incubator or water bath and mix gently by inversion after every 10 min till 45 min. After incubation, place the tube at room temperature for five min to reach to room temperature environment. Centrifuge the 50ml falcon tube for 5 min at 3000×g on room temperature. For next generation sequencing, the greater the genome size, lower is speed of initial centrifugation" ;
    a pplan:Step ;
    bs:labEquipmentUsed obo:OBI_0000136 , obo:OBI_0400155 , <http://www.bioassayontology.org/bao#BAO_0010014> .
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "falcon tube" .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:44:41.382183"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:ZITP0oKvEeuHtUOXv-HIvw , authority:eP-43IKvEeu7mWs8IOuakQ , authority:jvVxXoKvEeuhtjd8PHYffg , authority:p66r6IKvEeu27_Nt-Serng .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "OPo15ZBaRBp4shoo+wu9RBtL6dqAcqABzkETwAkbMrs4j40IpsQ43WeZAfxZlOGA1yVTqtafhcH7p6XTX1eVW9Yq29v04ujPMBt9Pmaq/dzo8cn7XFkNWFOYJe8vEAXTXyU5C6zGjW5kQziZWCvxbksXRWlV4+jSgFIuZr0xJ6s=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:44:41.382183"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}