@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAGn8YBHlzkpcQoQ3d8Kk4SBJnpDp5vi9xmfKQNOF-0hw> .
@prefix sub: <http://purl.org/np/RAGn8YBHlzkpcQoQ3d8Kk4SBJnpDp5vi9xmfKQNOF-0hw#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "upper aqueous phase" .
  
  sub:_2 rdfs:label "tube (size= 1.5 ml)" .
  
  sub:step dce:description "Transfer the upper aqueous phase to a new tube (size= 1.5 ml)";
    a pplan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-13T15:42:40.695486"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:HgjmRnJIEeu05Ltj67z_KQ, authority:TbP5vHJIEeusaUsGHPa6kw,
      authority:enNSSnJIEeub8HdzXFzTbQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "R5lhVY8dB6Dp7ccTcZvbfaBF2PMvyohLVf2c1HRFddY+9wQekyN3RCmohKJ7mSrbdumCFpuSmpt2n3gdlEgVGcFhyLAB25lOUWtwI9M6OEOKg4yFucafBcL0W0ics66OBOfeCNp5KI+xWM1tuprX45Qo+j63G+7muGeh3Tul4kE=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-13T15:42:40.695486"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}