@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAL2z7_iCJ0JDOiPsud19GQIi0T-KqnE8hkqkRYNb5TQU> .
@prefix sub: <http://purl.org/np/RAL2z7_iCJ0JDOiPsud19GQIi0T-KqnE8hkqkRYNb5TQU#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:step dce:description "Lyse for 1 hour 30 minutes at 37º celcius. Gently mix the tube by inverting every 15 minutes.";
    a pplan:Step;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T10:51:04.012870"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:4UqHzIJ-Eeu4-dsf53mYkA, authority:yXR8NIJ-EeudOd8-zPRS2A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "MDdSi9L8FHBttQWYZJygJsW+ZlAj1v6bbdXkU+Lrdrz0WSKNXaVAnFmOoGhNn1Y9w440h7/DLAQQRktMOwfd4F0xQQpbea27fG+78p4Suo6cc7vjWIf1ddKLh/LmGsz68Jxapy3D5V7ar+tvD8w1peLyIQctefbo5w7L/DWlJD0=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T10:51:04.012870"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}