@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAMEWHL2VaXey4LGj9uqunry-i5DelMCHFXIRYE4cfkvk> .
@prefix sub: <http://purl.org/np/RAMEWHL2VaXey4LGj9uqunry-i5DelMCHFXIRYE4cfkvk#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "Cells" .
  
  sub:_2 rdfs:label "Cells" .
  
  sub:_3 rdfs:label "100-mm-diameter dishes" .
  
  sub:_4 rdfs:label "medium" .
  
  sub:_5 rdfs:label "IBV" .
  
  sub:step dce:description "Cells were seeded in 100-mm-diameter dishes and infected with either 2 PFU of live IBV per cell or the same amount of UV-inactivated IBV (UV-IBV) at 37 °C. Excess virus in the medium was removed by replacing with fresh medium at 1 h post-infection.";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed sub:_3;
    bs:reagentUsed sub:_4, sub:_5 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-18T09:39:44.521411"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:9wksFnE7EeuVIqvYfYWhcg, authority:V3A1mnE8EeuR5n_LDgp7nA,
      authority:Y22XbHE7EeuNu6ti6pzHHg, authority:vOG58nH2Eeu4X_NNN02iog, authority:v_WsVHE7EeuNwJMqEC032A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "S2mgQ9zBlPMcY6dQVord5NeZdTBMhEe5F1HqECaqeLlT5qgoSmbWYc3FpVRPh8Xj5dZQJ2c0qldnZpywee54M5Z3wLunrXHrsfCXqlgGiAmYzhkKpkof9wUVDPcMU1spKF0bMSvqV7fBek7imIXMRSAykGPKQaX8+idqRKgo6dc=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-18T09:39:44.521411"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}