@prefix obo: .
@prefix dce: .
@prefix pplan: .
@prefix this: .
@prefix sub: .
@prefix bs: .
@prefix np: .
@prefix pav: .
@prefix authority: .
@prefix npx: .
@prefix xsd: .
@prefix rdfs: .
@prefix prov: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
}
sub:assertion {
obo:OBI_0400155 rdfs:label "water bath" .
sub:_1 rdfs:label "suspension" .
sub:_2 rdfs:label "100 mg/ml lysozyme" .
sub:step dce:description """Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""";
a pplan:Step;
bs:bioSampleUsed sub:_1;
bs:labEquipmentUsed obo:OBI_0400155;
bs:reagentUsed sub:_2 .
}
sub:provenance {
sub:assertion pav:createdWith ;
prov:generatedAtTime "2021-03-10T11:18:25.462488"^^xsd:dateTime;
prov:wasAttributedTo .
}
sub:pubInfo {
sub:assertion prov:wasDerivedFrom authority:EF-yuIFSEeuet6vjBV_k6Q, authority:JfaRoIFSEeu374uFEHtqAg,
authority:K0yIKoG8EeujNmf67OBRmw, authority:tmG8cIFREeuamj8DU21Bpw .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
npx:hasSignature "AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8=";
npx:hasSignatureTarget this: .
this: npx:introduces sub:step;
prov:generatedAtTime "2021-03-10T11:18:25.462488"^^xsd:dateTime;
prov:wasAttributedTo .
}