@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAMgSaFs7LsNJajmKEtzhd92lc4PAX4U3obVYFL-3ZIHk> .
@prefix sub: <http://purl.org/np/RAMgSaFs7LsNJajmKEtzhd92lc4PAX4U3obVYFL-3ZIHk#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:step dce:description "Centrifuge at 13700 g at 4℃ for 10 min.CRITICAL STEP The PH of the extraction buffer is about 8-9, resulting in DNA and RNA precipitation, however, it could result in lower DNA and higher RNA concentrations in case of reducing the PH to around 6-7.";
    a pplan:Step .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T11:39:12.571035"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:_pC7yH3HEeuGhXesFmOlCw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "F81BciwYkthAA44Yb07IUcPs3H7qFbRejFUazhMg+ler20hM7vP3S+psGTFTv/a2Dh5YVd0/kGJzKSkGh4L+zcc7i/G7gfMBQzXZS4nFuQ+yzh/NBxVMKzE/LKzWzsZT10HcAVUUpmMrss5PipyKWNeovuOQYWG05LzvG+8V0ko=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T11:39:12.571035"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}