http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#Head http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#provenance http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#pubinfo http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:98254 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:96790 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_3 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_2 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_1 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.w3.org/2000/01/rdf-schema#label p(MGI:Scp2) -| p(MGI:Lipe) http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_4 http://www.w3.org/ns/prov#value Despite the critical role lipid droplets play in maintaining energy reserves and lipid stores for the cell, little is known about the regulation of the lipid or protein components within the lipid droplet. Although immunofluorescence of intact cells as well as Western analysis of isolated lipid droplets revealed that sterol carrier protein-2 (SCP-2) was not associated with lipid droplets, SCP-2 expression significantly altered the structure of the lipid droplet. First, the targeting of fatty acid and cholesterol to the lipid droplets was significantly decreased. Second, the content of several proteins important for lipid droplet function was differentially increased (perilipin A), reduced severalfold (adipose differentiation-related protein (ADRP), vimentin), or almost completely eliminated (hormone-sensitive lipase and proteins >93 kDa) in the isolated lipid droplet. Third, the distribution of lipids within the lipid droplets was significantly altered. Double labeling of cells with 12-(N-methyl)-N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-octadecanoic acid (NBD-stearic acid) and antisera to ADRP showed that 70, 24, and 13% of lipid droplets contained ADRP, NBD-stearic acid, or both, respectively. SCP-2 expression decreased the level of ADRP in the lipid droplet but increased the proportion wherein ADRP and NBD-stearic acid colocalized by 3-fold. SCP-2 expression also decreased the lipid droplet fatty acid and cholesterol mass (nmol/mg protein) by 5.2- and 6.6-fold, respectively. Finally, SCP-2 expression selectively altered the pattern of esterified fatty acids in favor of polyunsaturated fatty acids within the lipid droplet. Displacement studies showed differential binding affinity of ADRP for cholesterol and fatty acids. These data suggested that SCP-2 and ADRP play a significant role in regulating fatty acid and cholesterol targeting to lipid droplets as well as in determining their lipid and protein components. http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11333258 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11333258 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#_4 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8#pubinfo http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://purl.org/dc/terms/created 2014-07-03T14:29:55.971+02:00 http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAQNp4kLTzxVfP_5xDDP6Ihn3iCaK3Kpti71rUyNhfeW8 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234