sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_4 ;
    pav:version "1.4" .
  sub:_3 prov:value "CORTICOTROPIN regulates the steroidogenic capacity, size, and structural integrity of the adrenal cortex through a series of actions involving changes in gene expression; however, only a limited number of CORTICOTROPIN-regulated genes have been identified, and these only partly account for the global effects of CORTICOTROPIN on the adrenal cortex. In this study, a National Institute on Aging 15K mouse cDNA microarray was used to identify genome-wide changes in gene expression after treatment of Y1 mouse adrenocortical cells with CORTICOTROPIN. CORTICOTROPIN affected the levels of 1275 annotated transcripts, of which 46% were up-regulated. The up-regulated transcripts were enriched for functions associated with steroid biosynthesis and metabolism; the down- regulated transcripts were enriched for functions associated with cell proliferation, nuclear transport and RNA processing, including alternative splicing. A total of 133 different transcripts, i.e. only 10% of the CORTICOTROPIN-affected transcripts, were represented in the categories above; most of these had not been described as CORTICOTROPIN-regulated previously. The contributions of protein kinase A and protein kinase C to these genome-wide effects of CORTICOTROPIN were evaluated in microarray experiments after treatment of Y1 cells and derivative protein kinase A-defective mutants with pharmacological probes of each pathway. Protein kinase A-dependent signaling accounted for 56% of the CORTICOTROPIN effect; protein kinase C-dependent signaling accounted for an additional 6%. These results indicate that CORTICOTROPIN affects the expression profile of Y1 adrenal cells principally through cAMP- and protein kinase A- dependent signaling. The large number of transcripts affected by CORTICOTROPIN anticipates a broader range of actions than previously appreciated." ;
    prov:wasQuotedFrom pubmed:16484322 .
  sub:_4 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:16484322 ;
    prov:wasDerivedFrom beldoc: , sub:_3 .
}