. . . . . . . . . . . . "a(CHEBI:corticotropin) -> r(MGI:Star)" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "1.4" . "CORTICOTROPIN regulates the steroidogenic capacity, size, and structural integrity of the adrenal cortex through a series of actions involving changes in gene expression; however, only a limited number of CORTICOTROPIN-regulated genes have been identified, and these only partly account for the global effects of CORTICOTROPIN on the adrenal cortex. In this study, a National Institute on Aging 15K mouse cDNA microarray was used to identify genome-wide changes in gene expression after treatment of Y1 mouse adrenocortical cells with CORTICOTROPIN. CORTICOTROPIN affected the levels of 1275 annotated transcripts, of which 46% were up-regulated. The up-regulated transcripts were enriched for functions associated with steroid biosynthesis and metabolism; the down- regulated transcripts were enriched for functions associated with cell proliferation, nuclear transport and RNA processing, including alternative splicing. A total of 133 different transcripts, i.e. only 10% of the CORTICOTROPIN-affected transcripts, were represented in the categories above; most of these had not been described as CORTICOTROPIN-regulated previously. The contributions of protein kinase A and protein kinase C to these genome-wide effects of CORTICOTROPIN were evaluated in microarray experiments after treatment of Y1 cells and derivative protein kinase A-defective mutants with pharmacological probes of each pathway. Protein kinase A-dependent signaling accounted for 56% of the CORTICOTROPIN effect; protein kinase C-dependent signaling accounted for an additional 6%. These results indicate that CORTICOTROPIN affects the expression profile of Y1 adrenal cells principally through cAMP- and protein kinase A- dependent signaling. The large number of transcripts affected by CORTICOTROPIN anticipates a broader range of actions than previously appreciated." . . "Selventa" . . . . "2014-07-03T14:30:41.612+02:00"^^ . . .