http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#Head http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#provenance http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#pubinfo http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.ebi.ac.uk/chebi/searchId.do?chebiId=26710 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=40919 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:aldosterone) -> a(CHEBI:"sodium chloride") http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_3 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_2 http://www.w3.org/ns/prov#value Escape from aldosterone-induced renal NaCl retention is an important homeostatic mechanism in pathophysiological states in which plasma aldosterone levels are inappropriately elevated, e.g., in primary aldosteronism. Our previous studies demonstrated that the escape process occurs largely as a result of a marked suppression of the abundance of the thiazide-sensitive Na-Cl cotransporter (NCC) of the distal convoluted tubule but have also demonstrated a paradoxical increase in the protein abundance of the apical Na/H exchanger of the proximal tubule (NHE3). In the present study, we confirmed the increase in NHE3 and also showed that a similar increase in NHE3 protein abundance occurs in escape from ANG II-mediated NaCl retention. To investigate the potential role of nitric oxide (NO) in the observed upregulation of NHE3, we repeated the aldosterone escape experiment with a superimposed infusion of a NO synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME). l-NAME infusion abolished the increase in NHE3 protein abundance. Furthermore, in a different experiment, NO synthase inhibition uncovered an associated decrease in the abundance of the Na-K-2Cl cotransporter (NKCC2) of the thick ascending limb, not seen with simple aldosterone escape. However, NO synthase inhibition did not block the decrease in NCC abundance normally seen with aldosterone escape. Furthermore, l-NAME infusion in aldosterone-treated rats markedly decreased both NHE3 and NKCC2 protein abundance, without changes in the corresponding mRNA levels. We conclude that NHE3 and NKCC2 protein abundances in kidney are positively regulated by NO and that the increase in NHE3 abundance seen in the aldosterone escape phenomenon is NO dependent. aldosterone-induced renal NaCl retention is an important homeostatic mechanism http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_2 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12837683 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_3 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12837683 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#_2 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0#pubinfo http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://purl.org/dc/terms/created 2014-07-03T14:30:14.126+02:00 http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RATKClv9H2t264z7KlT5bftLnd2mkJw14dL7H7V1Thev0 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234