http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#Head http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#provenance http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#pubinfo http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=12868 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D009000 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_1 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=7583 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:"nitric oxide") -> r(HGNC:ZFPL1) http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_3 http://www.w3.org/ns/prov#value Nitric oxide (NO*) can stabilize mRNA by activating p38 mitogen-activated protein kinase (MAPK). Here, transcript stabilization by NO* was investigated in human THP-1 cells using microarrays. After LPS pre-stimulation, cells were treated with actinomycin D and then exposed to NO* without or with the p38 MAPK inhibitor SB202190 (SB). The decay of 220 mRNAs was affected; most were stabilized by NO*. Unexpectedly, SB often enhanced rather than antagonized transcript stability. NO* activated p38 MAPK and Erk1/2; SB blocked p38 MAPK, but further activated Erk1/2. RT-PCR confirmed that NO* and SB could additively stabilize certain mRNA transcripts, an effect abolished by Erk1/2 inhibition. In affected genes, these responses were associated with CU-rich elements (CURE) in 3'-untranslated regions (3'-UTR). NO* stabilized the mRNA of a CURE-containing reporter gene, while repressing translation. Dominant-negative Mek1, an Erk1/2 inhibitor, abolished this effect. NO* similarly stabilized, but blocked translation of MAP3K7IP2, a natural CURE-containing gene. NO* increased hnRNP translocation to the cytoplasm and binding to CURE. Over-expression of hnRNP K, like NO*, repressed translation of CURE-containing mRNA. These findings define a sequence-specific mechanism of NO*-triggered gene regulation that stabilizes mRNA, but represses translation. http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16757573 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16757573 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#_3 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA#pubinfo http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://purl.org/dc/terms/created 2014-07-03T14:30:43.487+02:00 http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RATtq7jw_CzgQTKr1dEvrJJMGhjhcSPNHU0uUhePH5boA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234