http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#Head http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#provenance http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=25475 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D010053 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_1 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0006306 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GO:"DNA methylation") -| r(HGNC:BEX4) http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_3 http://www.w3.org/ns/prov#value Epigenetic silencing by hypermethylation of CpGs represents a mechanism of inactivation of tumor suppressors. Here we report on the cloning of a novel candidate tumor suppressor gene TCEAL7 inactivated by methylation in ovarian cancer. TCEAL codes for a 1.35 kb transcript that was previously reported to be downregulated in ovarian cancer by cDNA microarray and suppression subtraction cDNA (SSH) analyses. This report focuses on the elucidation of mechanisms associated with TCEAL7 downregulation. Expression of TCEAL7 is downregulated in a majority of ovarian tumors and cancer cell lines but induced by 5-aza-2'-deoxycytidine treatment in a dose-dependant manner, implicating methylation as a mechanism of TCEAL7 inactivation. Sequence analyses of bisufite-modified genomic DNA from somatic cell hybrids with either the active or the inactive human X chromosome reveal that TCEAL7 is subjected to X chromosome inactivation. Loss of TCEAL7 expression in primary tumors and cell lines correlates with methylation of a CpG site within the promoter. In vitro methylation of the CpG site suppresses promoter activity whereas selective demethylation of the SmaI site attenuates the suppression. Finally, re-expression of TCEAL7 in cancer cell lines induces cell death and reduces colony formation efficiency. These data implicate TCEAL7 as a cell death regulatory protein that is frequently inactivated in ovarian cancers, and suggest that it may function as a tumor suppressor. http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15870691 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15870691 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#_3 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://purl.org/dc/terms/created 2014-07-03T14:30:35.173+02:00 http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAUTtJUUcVTCED5ma6Z6ihbg3PMYGMWBY7DrXq5-yx1Lw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234