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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#_3 http://www.w3.org/ns/prov#value Lipopolysaccharide (LPS) activation of murine RAW 264.7 macrophages influences the expression of multiple genes through transcriptional and post-transcriptional mechanisms. We observed a 5-fold increase in CstF-64 expression following LPS treatment of RAW macrophages. The increase in CstF-64 protein was specific in that several other factors involved in 3'-end processing were not affected by LPS stimulation. Activation of RAW macrophages with LPS caused an increase in proximal poly(A) site selection within a reporter mini-gene containing two linked poly(A) sites that occurred concomitant with the increase in CstF-64 expression. Furthermore, forced overexpression of the CstF-64 protein also induced alternative poly(A) site selection on the reporter minigene. Microarray analysis performed on CstF-64 overexpressing RAW macrophages revealed that elevated levels of CstF-64 altered the expression of 51 genes, 14 of which showed similar changes in gene expression with LPS stimulation. Sequence analysis of the 3'-untranslated regions of these 51 genes revealed that over 45% possess multiple putative poly(A) sites. Two of these 51 genes demonstrated alternative polyadenylation under both LPS-stimulating and CstF-64-overexpressing conditions. We concluded that the physiologically increased levels of CstF-64 observed in LPS-stimulated RAW macrophages contribute to the changes in expression and alternative polyadenylation of a number of genes, thus identifying another level of gene regulation that occurs in macrophages activated with LPS. http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16207706 http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16207706 http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#_3 http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw http://purl.org/dc/terms/created 2014-07-03T14:30:38.601+02:00 http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAVQ6wHb9l-3r0GCGGQJ6PisjxVhnoTS7N74eIaYwIfjw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234