sub:provenance { beldoc:dce:description "Approximately 61,000 statements." ; dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ; dce:title "BEL Framework Large Corpus Document" ; pav:authoredBysub:_6 ; pav:version "1.4" . sub:_5prov:value "PRAS40 was phosphorylated in vitro by purified Akt on the same site that was phosphorylated in insulin-treated cells. (From Results section) -- To determine whether the predicted Akt consensus phosphorylation site (Thr-246) was in fact the site recognized by the anti-pAkt substrate antibody, a mutant form of the HA-tagged PRAS40 (T246A) was expressed in which this site was changed to Ala. The anti-pAkt substrate did not recognize the mutant PRAS40, even when the constitutively active Akt was co-expressed with this protein (Fig. 5). --" ; prov:wasQuotedFrompubmed:12524439 . sub:_6rdfs:label "Selventa" . sub:assertionprov:hadPrimarySourcepubmed:12524439 ; prov:wasDerivedFrombeldoc: , sub:_5 . }