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http://purl.obolibrary.org/obo/RO_0002204
http://www.informatics.jax.org/marker/MGI:97747
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a(CHEBI:"carbon monoxide") -| r(MGI:Il6)
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p(MGI:Pparg) -> (a(CHEBI:"carbon monoxide") -| r(MGI:Il6))
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Approximately 61,000 statements.
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Copyright (c) 2011-2012, Selventa. All rights reserved.
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BEL Framework Large Corpus Document
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1.4
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Table S3: DNA microarray gene expression profile of LPS-induced gene expression in CO-exposed RAW 264.7 macrophages in the presence and absence of PPAR? RNA interference. Murine 430 2.0 GeneChips were used for screening PPARgamma-mediated effects on LPS-activaed RAW 264.7 macrophages in the absence or presence of CO (250 ppm; Figure 4D of the main text). Cells were exposed to 2-4 hr CO (250 ppm) or air as previously described (Otterbein et al., 2000) prior to addition of LPS (1 ng/ml E. coli serotype O127:B8; Sigma, St. Louis, MO). For PPARgamma knockdown assays, the medium was replaced 36 hr after transfection of the preannealed oligo, and cells were stimulated with LPS another 12 hr later in the absence or presence of CO. Data are representative of 3 independent samples. Statistical analysis was made by using Student's t test. Results were considered statistically significant at p < 0.05.
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Selventa
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2014-07-03T14:30:43.190+02:00
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