@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAXD6cg3fHQTYs2Q0oZyWKeYPNCK6durVr_TBpqqp-86s> .
@prefix sub: <http://purl.org/np/RAXD6cg3fHQTYs2Q0oZyWKeYPNCK6durVr_TBpqqp-86s#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sp:RNAPellet rdfs:label "RNA pellet" .
  
  sub:_1 rdfs:label "70% RNase-free ethanol " .
  
  sub:step dce:description "RNA pellet was washed with 1 ml 70% RNase-free ethanol once and spin down by 7,500 x g for 5 min";
    sp:hasExperimentalOutput sp:RNAPellet;
    a pplan:Step;
    bs:bioSampleUsed sp:RNAPellet;
    bs:reagentUsed sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-18T09:59:20.343537"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:2WC2bHE_Eeu7msMdB-ko7Q, authority:_arkonE_EeuO3aPu4noR8A,
      authority:zMWFlHH5Eeup9H8m2uTkPQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "PfQOvXRio6vGXygz/Pri1AMNrpG30wZPy21vevo45NC5r7DqmRwM68CraLuJH3AQ/RU24nf1yS95KaRjUc1yXhPgDyF/8BV0U3WMzlLTbZgWOTMaQwpeYSqAGo8+alPL0NEvsJro8glPUtzLg2qpTDkg5oeka0pD2eaVEZdQXUQ=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-18T09:59:20.343537"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}