http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#Head http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#provenance http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#pubinfo http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:97011 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D008264 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_1 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=6494 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:lipopolysaccharide) -> r(MGI:Mmp9) http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_3 http://www.w3.org/ns/prov#value Lipopolysaccharide (LPS) activation of murine RAW 264.7 macrophages influences the expression of multiple genes through transcriptional and post-transcriptional mechanisms. We observed a 5-fold increase in CstF-64 expression following LPS treatment of RAW macrophages. The increase in CstF-64 protein was specific in that several other factors involved in 3'-end processing were not affected by LPS stimulation. Activation of RAW macrophages with LPS caused an increase in proximal poly(A) site selection within a reporter mini-gene containing two linked poly(A) sites that occurred concomitant with the increase in CstF-64 expression. Furthermore, forced overexpression of the CstF-64 protein also induced alternative poly(A) site selection on the reporter minigene. Microarray analysis performed on CstF-64 overexpressing RAW macrophages revealed that elevated levels of CstF-64 altered the expression of 51 genes, 14 of which showed similar changes in gene expression with LPS stimulation. Sequence analysis of the 3'-untranslated regions of these 51 genes revealed that over 45% possess multiple putative poly(A) sites. Two of these 51 genes demonstrated alternative polyadenylation under both LPS-stimulating and CstF-64-overexpressing conditions. We concluded that the physiologically increased levels of CstF-64 observed in LPS-stimulated RAW macrophages contribute to the changes in expression and alternative polyadenylation of a number of genes, thus identifying another level of gene regulation that occurs in macrophages activated with LPS. http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16207706 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16207706 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#_3 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI#pubinfo http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://purl.org/dc/terms/created 2014-07-03T14:30:38.621+02:00 http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAZY4O-iuSJby74cR8NdlcqqnbTg2S_jk-CQL-PC1bmNI http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234