sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_4 ;
    pav:version "1.4" .
  sub:_3 prov:value "In contrast to Bid, the antiapoptotic members of the Bcl-2 protein family, such as Bcl-2 and Bcl-xL, are known to preserve the integrity of the mitochondrial membrane (34). We thus explored whether the Siva-induced apoptosis in T cells could be modulated by overexpression of Bcl-2 or Bcl-xL. Jurkat T cells overexpressing either Bcl-2 or Bcl-xL were transiently transfected with the GFP-Siva-1 expression vector, and cell surface PS exposure was analyzed 48 h later on GFP-positive cells (Fig. 5C). Expression of GFP-Siva-1 in control Jurkat cells stably transfected with the neomycin vector resulted in an apoptotic level absolutely similar to that observed in HPB-ALL T cells. In contrast, the percentage of GFP-Siva-1-expressing cells displaying a cell surface exposure of PS was reduced to a level corresponding to the GFP control in cells coexpressing Bcl-2 or Bcl-xL. These results show that the proapoptotic activity of GFP-Siva-1 in Jurkat T cells can be totally abrogated by overexpression of either Bcl-2 or Bcl-xL antiapoptotic proteins. Because studies on the Fas-mediated apoptosis signaling pathways have highlighted two types of cells (35) depending on the requirement (type II cells such as Jurkat cells) or not (type I cells) of a mitochondrial amplification loop, we explored the Siva-induced apoptosis in a type I cell line such as SKW6.4 B lymphoid cells overexpressing Bcl-2 (35). In contrast to Jurkat cells, overexpression of Bcl-2 in SKW6.4 cells only reduced by 50% the proportion of apoptotic GFP-Siva-1-expressing cells (Fig. 5D). This result indicates that the Siva-induced apoptosis is also, at least in part, modulated by antiapoptotic members of the Bcl-2 protein family in type I cells. (From full text)" ;
    prov:wasQuotedFrom pubmed:15034012 .
  sub:_4 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:15034012 ;
    prov:wasDerivedFrom beldoc: , sub:_3 .
}