http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#Head http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#provenance http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=6363 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_1 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=50113 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:androgen) -> r(HGNC:KLK2) http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_3 http://www.w3.org/ns/prov#value Accumulating evidence indicates that androgens and the androgen receptor modulate the development and progression of breast adenocarcinoma; however, the precise role and actions remain poorly defined. We examined previously the steroid hormone regulation of 2 known androgen-regulated kallikreins, KLK3 (encoding PSA) and KLK2 (encoding human kallikrein 2 or hK2) in BT-474, T-47D, ZR75-1, MCF-7, MFM-223 and BT-20 human breast cancer cells and found that they were differentially regulated, with the cells showing variable responses to androgen. To determine if this variable response was reflected by differences in androgen receptor, we characterized the expression of androgen receptor in these cells by Western blot analysis and saturation binding analysis. In addition, we sequenced androgen receptor cDNA from each of these cell lines to check whether any androgen receptor mutations were present. The expression of 11 nuclear receptor co-regulatory factors (SRC-1, AIB1, ARA24, ARA54, ARA55, ARA70, ARA160, FHL2, PDEF, NCoR1, SMRT) was compared in these cell lines by semi-quantitative RT-PCR to determine if the pattern of receptor co-activators or -repressors expressed in these cells might explain the differential regulation of KLK2 and KLK3. The levels of androgen receptor varied among the cell lines, but did not correlate with hK2 and PSA secretion determined previously. No mutations within the coding regions of the receptor were detected. With the exception of receptor expressed by MCF-7 cells, the polymorphic CAG repeat length was in the normal range. Every breast cancer cell line exhibited a distinct expression pattern of the nuclear receptor co-regulators examined raising the possibility that the relative levels of these co-activators/-repressors might differentially modulate androgen receptor transcriptional activity within the promoter/enhancer region of KLK2 and KLK3 of these cells. http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12124798 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12124798 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#_3 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://purl.org/dc/terms/created 2014-07-03T14:30:04.469+02:00 http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAaCcPn54ZmtgNL59I5X-stk7kW4asI_QSUyLL4VE7-XU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234