. . . . . . . . . . . "a(CHEBI:\"25-hydroxycholesterol\") -> r(HGNC:OSBPL1A)" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "1.4" . "Culturing the cells in lipoprotein-deficient serum for 2 days decreased their total cholesterol concentration by 25% (from 68 to 51ug cholesterol/mg protein) but did not significantly alter the ORP-1 mRNA levels. However, when the partially delipidated cells were loaded with LDL for 24 h, the ORP-1 transcript was up-regulated by 2-fold (Fig. 5). Simultaneously, the cellular cholesterol level returned back to normal (not shown). Short-term treatment of the sterol-depleted cells with 25-hydroxycholesterol (5 ug/ml of 25-hydroxycholesterol for 4 h), a condition known to stimulate the translocation of OSBP to the Golgi apparatus (10, 13), had a minor (1.5-fold) stimulatory effect on the expression of the ORP-1 mRNA (Fig. 5)." . . "Selventa" . . . . "2014-07-03T14:29:49.441+02:00"^^ . . .