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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#_4 http://www.w3.org/ns/prov#value even though this fusion protein efficiently prevented BrdU uptake in MCK-CAT-positive cells (Figure 4h-j). As E2F1-pRb(SP) retained the anti-proliferative property of pRb but did not fully activate myogenesis on its own, we tested whether it could cooperate with MEF2C-VP16 in our myogenesis assays. The combination of MyoD+E12, MEF2C-VP16, and E2F1-pRb(SP) activated MCK-luciferase expression to a level equal to or greater than that achieved following co-transfection of MyoD+E12 with pRb (Figure 4a, compare columns 3 and 7), and led to the appearance of numerous cells in the culture that displayed very high levels of CAT staining and no BrdU incorporation (Figure 4k-m), very similar to what was observed following transfection of MyoD with pRb (Figure 4b-d). Cotransfection of E2F1-pRb(SP) and MEF2C-VP16 in the absence of MyoD did not induce the expression of MCK-CAT (data not shown). Thus, we were able to fully substitute for the loss of pRb by cotransfecting MyoD with an activated form of MEF2 and the cell-cycle-arresting molecule E2F1-pRb(SP). http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10322110 http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10322110 http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#_4 http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw http://purl.org/dc/terms/created 2014-07-03T14:29:47.108+02:00 http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAc_MWfVT-8wVAcrrm32hhGKqC_dnHlvBLiCbt7zF9zuw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234