[ {
  "@graph" : [ {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes",
    "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ],
    "http://www.nanopub.org/nschema#hasAssertion" : [ {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#assertion"
    } ],
    "http://www.nanopub.org/nschema#hasProvenance" : [ {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#provenance"
    } ],
    "http://www.nanopub.org/nschema#hasPublicationInfo" : [ {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#pubinfo"
    } ]
  } ],
  "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#Head"
}, {
  "@graph" : [ {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_1",
    "@type" : [ "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697" ],
    "http://purl.obolibrary.org/obo/RO_0002204" : [ {
      "@id" : "http://www.informatics.jax.org/marker/MGI:95295"
    } ]
  }, {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_2",
    "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ],
    "http://purl.obolibrary.org/obo/BFO_0000066" : [ {
      "@id" : "http://purl.bioontology.org/ontology/MSH/D005347"
    }, {
      "@id" : "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090"
    } ],
    "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_1"
    } ],
    "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ {
      "@id" : "http://www.selventa.com/vocabulary/increases"
    } ],
    "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ {
      "@id" : "http://resource.belframework.org/belframework/1.0/namespace/selventa-legacy-chemical-names/serum%20insulin"
    } ]
  }, {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#assertion",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "a(SCHEM:\"serum insulin\") -> r(MGI:Egr1)"
    } ]
  } ],
  "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#assertion"
}, {
  "@graph" : [ {
    "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel",
    "http://purl.org/dc/elements/1.1/description" : [ {
      "@value" : "Approximately 61,000 statements."
    } ],
    "http://purl.org/dc/elements/1.1/rights" : [ {
      "@value" : "Copyright (c) 2011-2012, Selventa. All rights reserved."
    } ],
    "http://purl.org/dc/elements/1.1/title" : [ {
      "@value" : "BEL Framework Large Corpus Document"
    } ],
    "http://purl.org/pav/authoredBy" : [ {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_4"
    } ],
    "http://purl.org/pav/version" : [ {
      "@value" : "1.4"
    } ]
  }, {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_3",
    "http://www.w3.org/ns/prov#value" : [ {
      "@value" : "The human insulin receptor (IR) exists in two isoforms (IR-A and IR-B). IR-A is a short isoform, generated by the skipping of exon 11, a small exon encoding for 12 amino acid residues at the carboxyl terminus of the IR alpha-subunit. Recently, we found that IR-A is the predominant isoform in fetal tissues and malignant cells and binds with a high affinity not only insulin but also insulin-like growth factor-II (IGF-II). To investigate whether the activation of IR-A by the two ligands differentially activate post-receptor molecular mechanisms, we studied gene expression in response to IR-A activation by either insulin or IGF-II, using microarray technology. To avoid the interfering effect of the IGF-IR, IGF-II binding to the IR-A was studied in IGF-IR-deficient murine fibroblasts (R- cells) transfected with the human IR-A cDNA (R-/IR-A cells). Gene expression was studied at 0.5, 3, and 8 h. We found that 214 transcripts were similarly regulated by insulin and IGF-II, whereas 45 genes were differentially transcribed. Eighteen of these differentially regulated genes were responsive to only one of the two ligands (12 to insulin and 6 to IGF-II). Twenty-seven transcripts were regulated by both insulin and IGF-II, but a significant difference between the two ligands was present at least in one time point. Interestingly, IGF-II was a more potent and/or persistent regulator than insulin for these genes. Results were validated by measuring the expression of 12 genes by quantitative real-time reverse transcriptase-PCR. In conclusion, we show that insulin and IGF-II, acting via the same receptor, may differentially affect gene expression in cells. These studies provide a molecular basis for understanding some of the biological differences between the two ligands and may help to clarify the biological role of IR-A in embryonic/fetal growth and the selective biological advantage that malignant cells producing IGF-II may acquire via IR-A overexpression."
    } ],
    "http://www.w3.org/ns/prov#wasQuotedFrom" : [ {
      "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/12881524"
    } ]
  }, {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_4",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "Selventa"
    } ]
  }, {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#assertion",
    "http://www.w3.org/ns/prov#hadPrimarySource" : [ {
      "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/12881524"
    } ],
    "http://www.w3.org/ns/prov#wasDerivedFrom" : [ {
      "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel"
    }, {
      "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#_3"
    } ]
  } ],
  "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#provenance"
}, {
  "@graph" : [ {
    "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes",
    "http://purl.org/dc/terms/created" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2014-07-03T14:30:14.956+02:00"
    } ],
    "http://purl.org/pav/createdBy" : [ {
      "@id" : "http://orcid.org/0000-0001-6818-334X"
    }, {
      "@id" : "http://orcid.org/0000-0002-1267-0234"
    } ]
  } ],
  "@id" : "http://www.tkuhn.ch/bel2nanopub/RAclys_jJeAqY97gF95D2dhTnlK5xml09QJM62RGcIWes#pubinfo"
} ]