[ { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#pubinfo" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#Head" }, { "@graph" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/namespace/selventa-named-human-complexes/p85%2Fp110%20PI3Kinase%20Complex", "@type" : [ "http://amigo.geneontology.org/amigo/term/GO:0043234" ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_1", "@type" : [ "http://amigo.geneontology.org/amigo/term/GO:0016301" ], "http://semanticscience.org/resource/SIO_000139" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_2" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_2", "@type" : [ "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080" ], "http://purl.obolibrary.org/obo/RO_0002204" : [ { "@id" : "http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=6307" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_3", "@type" : [ "http://amigo.geneontology.org/amigo/term/GO:0016301" ], "http://semanticscience.org/resource/SIO_000139" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/namespace/selventa-named-human-complexes/p85%2Fp110%20PI3Kinase%20Complex" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_4", "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ], "http://purl.obolibrary.org/obo/BFO_0000066" : [ { "@id" : "http://purl.bioontology.org/ontology/MSH/D004730" }, { "@id" : "http://purl.bioontology.org/ontology/MSH/D042783" }, { "@id" : "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_3" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ { "@id" : "http://www.selventa.com/vocabulary/increases" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_1" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "kin(p(HGNC:KDR)) -> kin(complex(NCH:\"p85/p110 PI3Kinase Complex\"))" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion" }, { "@graph" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel", "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "Approximately 61,000 statements." } ], "http://purl.org/dc/elements/1.1/rights" : [ { "@value" : "Copyright (c) 2011-2012, Selventa. All rights reserved." } ], "http://purl.org/dc/elements/1.1/title" : [ { "@value" : "BEL Framework Large Corpus Document" } ], "http://purl.org/pav/authoredBy" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_6" } ], "http://purl.org/pav/version" : [ { "@value" : "1.4" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_5", "http://www.w3.org/ns/prov#value" : [ { "@value" : "The beta3 integrin is necessary for the activation of PI 3-kinase in endothelial cells stimulated by VEGF-A165 PI 3-kinase belongs to the intracellular signals triggered by VEGF-A in bovine endothelial cells, as demonstrated by the phosphorylation of its regulatory protein p85 (Guo et al., 1995). As shown in Figure 5, wortmannin used at nanomolar concentrations specific for PI 3-kinase inhibition (Arcaro and Wymann, 1993), reduced the human endothelial cells migration induced by VEGF-A165. To determine whether beta3 integrin interferes in PI 3-kinase activation occurring after VEGFR-2 stimulation, we evaluated the effect of BV4 (anti-beta3 subunit mAb) on the phosphorylation in tyrosine residues of the p85 subunit and on the catalytic activity of the enzyme. After pre-incubation with BV4 or with anti-VEGFR-2 Ab for 20 min at 4°C, cells were stimulated with VEGF-A165. Cell lysates were immunoprecipitated with anti-p85 mAb, and the separated proteins by SDS–PAGE were probed with anti-phosphotyrosine mAb. VEGF-A165 increased the tyrosine phosphorylation of p85, but it failed to phosphorylate the PI 3-kinase subunit when endothelial cells were pre-incubated with anti-beta3 or anti-VEGFR-2 antibodies (Figure 6)." } ], "http://www.w3.org/ns/prov#wasQuotedFrom" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/10022831" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_6", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "Selventa" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion", "http://www.w3.org/ns/prov#hadPrimarySource" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/10022831" } ], "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel" }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_5" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#provenance" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA", "http://purl.org/dc/terms/created" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2014-07-03T14:29:45.737+02:00" } ], "http://purl.org/pav/createdBy" : [ { "@id" : "http://orcid.org/0000-0001-6818-334X" }, { "@id" : "http://orcid.org/0000-0002-1267-0234" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#pubinfo" } ]