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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_5 http://www.w3.org/ns/prov#value The beta3 integrin is necessary for the activation of PI 3-kinase in endothelial cells stimulated by VEGF-A165 PI 3-kinase belongs to the intracellular signals triggered by VEGF-A in bovine endothelial cells, as demonstrated by the phosphorylation of its regulatory protein p85 (Guo et al., 1995). As shown in Figure 5, wortmannin used at nanomolar concentrations specific for PI 3-kinase inhibition (Arcaro and Wymann, 1993), reduced the human endothelial cells migration induced by VEGF-A165. To determine whether beta3 integrin interferes in PI 3-kinase activation occurring after VEGFR-2 stimulation, we evaluated the effect of BV4 (anti-beta3 subunit mAb) on the phosphorylation in tyrosine residues of the p85 subunit and on the catalytic activity of the enzyme. After pre-incubation with BV4 or with anti-VEGFR-2 Ab for 20 min at 4°C, cells were stimulated with VEGF-A165. Cell lysates were immunoprecipitated with anti-p85 mAb, and the separated proteins by SDS–PAGE were probed with anti-phosphotyrosine mAb. VEGF-A165 increased the tyrosine phosphorylation of p85, but it failed to phosphorylate the PI 3-kinase subunit when endothelial cells were pre-incubated with anti-beta3 or anti-VEGFR-2 antibodies (Figure 6). http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10022831 http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10022831 http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#_5 http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA#pubinfo http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA http://purl.org/dc/terms/created 2014-07-03T14:29:45.737+02:00 http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAdMwlC9OUw7nXQ-Ct-6XnNIv79Bh8j2IVKS6YNOoRhjA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234