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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#_4 http://www.w3.org/ns/prov#value U2AF is a heterodimeric splicing factor composed of a large (U2AF(65)) and a small (U2AF(35)) subunit. In humans, alternative splicing generates two U2AF(35) variants, U2AF(35)a and U2AF(35)b. Here, we used RNA interference to specifically ablate the expression of each isoform in HeLa cells. Our results show that knockdown of the major U2AF(35)a isoform reduced cell viability and impaired mitotic progression, leading to accumulation of cells in prometaphase. Microarray analysis revealed that knockdown of U2AF(35)a affected the expression level of approximately 500 mRNAs, from which 90% were underrepresented relative to the control. Among mRNAs underrepresented in U2AF(35)a-depleted cells we identified an essential cell cycle gene, Cdc27, for which there was an increase in the ratio between unspliced and spliced RNA and a significant reduction in protein level. Furthermore, we show that depletion of either U2AF(35)a or U2AF(35)b altered the ratios of alternatively spliced isoforms of Cdc25B and Cdc25C transcripts. Taken together our results demonstrate that U2AF(35)a is essential for HeLa cell division and suggest a novel role for both U2AF(35) protein isoforms as regulators of alternative splicing of a specific subset of genes. http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16855028 http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16855028 http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#_4 http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM#pubinfo http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM http://purl.org/dc/terms/created 2014-07-03T14:30:45.268+02:00 http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAe6df5OfOVVnOelQAYL7GSR5olbepBxZLr99KqnmHkCM http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234