http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#Head http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#provenance http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=7547 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.lgcstandards-atcc.org/Products/All/HTB-22.aspx http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_1 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=23965 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:estradiol) -> r(HGNC:MYBL1) http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_3 http://www.w3.org/ns/prov#value Antiestrogens and aromatase inhibitors are important drugs in the treatment of estrogen-dependent breast cancer. To investigate the effects of these drugs on gene expression in breast cancer cells, we treated estrogen receptor-positive MCF-7 cells stably transfected with the aromatase gene (known as MCF-7aro cells) with testosterone, 17 beta-estradiol, two aromatase inhibitors (letrozole and anastrozole), and an antiestrogen (tamoxifen). We found that testosterone or 17 beta-estradiol induced the proliferation of MCF-7aro cells at a rate six times faster than the untreated cells. In addition, the testosterone-induced proliferation of MCF-7aro cells was effectively suppressed by letrozole, anastrozole, or tamoxifen. Microarray analyses on Affymetrix Human Genome U133A GeneChips (Affymetrix, Santa Clara, CA) were carried out using total RNA isolated from the control and treated cells. At the false discovery rate of 0.05 and a minimum fold-change criteria of 1.5, 104 genes were identified that were up-regulated and 109 genes were identified that were down-regulated by both androgen and estrogen. More than 50% of these hormone-regulated genes were counter-regulated by all three inhibitors and >90% were counter-regulated by at least one of the inhibitors. Comparing the effect of each inhibitor on gene expression, we observed that letrozole and anastrozole are more similar in terms of the genes they affect compared with treatment with tamoxifen. To validate the gene expression profiles identified from microarray analyses, the expression patterns of 13 representative genes were examined by Northern analysis. Finally, the genes identified as statistically significant were classified based on their expression patterns and biological function/pathways. The results of this study provide us with a better understanding of the actions of both aromatase inhibitors and antiestrogens at the molecular level. We believe that the results of this study serve as the first step in identifying unique expression patterns following drug treatment, and that this will ultimately be useful in customizing patient treatment strategies for hormone-dependent breast cancer. http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15831674 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15831674 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#_3 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw#pubinfo http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://purl.org/dc/terms/created 2014-07-03T14:30:34.071+02:00 http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAeeADreokHIZgzVjPYlo6Tbx8MYX9NK3PYOoP-6IReLw http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234