http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#Head http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#provenance http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#pubinfo http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=5464 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_1 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=8453 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:progesterone) -| r(HGNC:IGF1) http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_3 http://www.w3.org/ns/prov#value BACKGROUND: Insulin-like growth factor-I (IGF-I) plays crucial roles in uterine leiomyoma cell growth through stimulating proliferation and inhibiting apoptosis. The present study was conducted to elucidate whether progesterone affects IGF-I and its receptor expression in cultured leiomyoma cells. METHODS: Isolated leiomyoma cells were subcultured in Phenol Red-free Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum for 120 h and then stepped down to serum-free conditions for an additional 48 h in the presence or absence of 17beta-estradiol (E(2)) (10 ng/ml) or progesterone (100 ng/ml). IGF-I and its receptor mRNA and immunoreactive IGF-I in the cultured cells were assessed by quantitative RT-PCR with Southern blot analysis and by radioimmunoassay with Seppak C18 chromatography, respectively. The presence of estrogen receptor (ER) and progesterone receptor (PR) in cultured leiomyoma cells was immunocytochemically examined. RESULTS: Both treatment with progesterone alone and treatment with E(2) and progesterone combined significantly decreased IGF-I mRNA and protein expression in cultured leiomyoma cells compared with that in untreated cultures, but treatment with E(2) alone did not. IGF-I receptor mRNA expression in those cells was not affected by treatment with either E(2) or progesterone. Immunocytochemical analysis revealed that PR protein expression in cultured leiomyoma cells maintained in a serum-free condition for 48 h whereas ER protein expression in the cells remarkably decreased after 24 h culture under the serum-free condition. CONCLUSIONS: The present study provided evidence for the first time that progesterone down-regulates IGF-I mRNA and protein expression in cultured leiomyoma cells without affecting IGF-I receptor mRNA expression. http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15033949 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15033949 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#_3 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk#pubinfo http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://purl.org/dc/terms/created 2014-07-03T14:30:21.707+02:00 http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAelLz3wZSb-Wn2GrkOtN7VCfPo6opr98FdTWSKs7MjZk http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234