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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#_3 http://www.w3.org/ns/prov#value The suppressor of cytokine signalling (SOCS) protein family negatively regulates cytokine action. In this study, we investigated the effects of estrogen (E2) on SOCS-3 expression in T47D and MCF-7 human breast cancer cells. Real-time PCR analysis of E2-treated T47D cells revealed a ligand and time-dependent increase in of SOCS-3 mRNA levels. Cloning of a 1.7 kb fragment of the human SOCS-3 5' flanking sequence, and subsequent analysis of potential transcription factor-binding sites identified an incomplete ERE motif located -1493 to -1489 upstream of the start site. Transient transfection of the cloned fragment in MCF-7 cells showed that both E2 and genistein treatment caused an increase in reporter gene activity, which was inhibited by co-treatment with ICI 182,780. Chromatin immunoprecipitation analysis revealed an E2 and time-dependent recruitment of ERalpha to the E2 responsive region of the human SOCS-3 promoter. In summary, this study shows that ERalpha directly regulates human SOCS-3 promoter activity in human breast cancer cells, thus modulating cytokine activity. http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16055089 http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16055089 http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#_3 http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA#pubinfo http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA http://purl.org/dc/terms/created 2014-07-03T14:30:37.040+02:00 http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAfTPeV-HrPsC4lIiKIutJaOv1NsIFgnqihdAN1u0mxJA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234