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Approximately 61,000 statements.
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Copyright (c) 2011-2012, Selventa. All rights reserved.
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We found that rictor immunopurifications contain isoforms 1, 2, and 5 (Figure 2C, left panel). mTOR immunopurifications also contain each of these isoforms (data not shown), whereas raptor immunopurifications do not (Figure 2C, right panel). In agreement with these findings, immunopurifications of myc-tagged isoforms 1, 2, and 5 coimmunoprecipitate mTOR and rictor but not raptor (Figure 2D, left panels). Knockdown of mSin1 with these two different shRNAs severely inhibited the phosphorylation of Ser473-Akt/PKB without affecting that of Thr389 of S6K1. We observed the same effect by knocking down rictor, whereas a control shRNA had no effect on Akt/PKB.
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2014-07-03T14:30:45.625+02:00
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