http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#Head http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#provenance http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=11892 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_2 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_3 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0042789 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_3 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=7794 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_4 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_2 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_1 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.w3.org/2000/01/rdf-schema#label p(HGNC:TNF) -> tscript(p(HGNC:NFKB1)) http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_5 http://www.w3.org/ns/prov#value This work presents direct evidence that the bcl-2 gene is transcriptionally regulated by nuclear factor-kappa B (NF-kappa B) and directly links the TNF-alpha/NF-kappa B signaling pathway with Bcl-2 expression and its pro-survival response in human prostate carcinoma cells. DNase I footprinting, gel retardation and supershift analysis identified a NF-kappa B site in the bcl-2 p2 promoter. In the context of a minimal promoter, this bcl-2 p2 site 1 increased transcription 10-fold in the presence of the p50/p65 expression vectors, comparable to the increment observed with the consensus NF-kappa B site, while for the full p2 promoter region transcriptional activity was increased sixfold by over-expression of NF-kappa B, an effect eliminated by mutating the bcl-2 p2 site 1. The expression of Bcl-2 has been linked to the hormone-resistant phenotype of advanced prostate cancer. Here we show that an increase in the level of expression of Bcl-2 in the human prostate carcinoma cell line LNCaP observed in response to hormone withdrawal is further augmented by TNF-alpha treatment, and this effect is abated by inhibitors of NF-kappa B. Concomitantly, bcl-2 p2 promoter studies in LNCaP cells show a 40-fold increase in promoter activity after stimulation with TNF-alpha in the absence of hormone. http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11704864 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11704864 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#_5 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://purl.org/dc/terms/created 2014-07-03T14:29:59.043+02:00 http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAg1Baa70ZVCvSFmXrFOGDZhmp6N1tEgAuc-EhgIyRkOU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234