@prefix this: . @prefix sub: . @prefix beldoc: . @prefix rdfs: . @prefix rdf: . @prefix xsd: . @prefix dct: . @prefix dce: . @prefix pav: . @prefix np: . @prefix belv: . @prefix prov: . @prefix Protein: . @prefix hgnc: . @prefix geneProductOf: . @prefix go: . @prefix nch: . @prefix ProteinComplex: . @prefix hasAgent: . @prefix species: . @prefix occursIn: . @prefix atcc: . @prefix pubmed: . @prefix orcid: . sub:Head { this: np:hasAssertion sub:assertion; np:hasProvenance sub:provenance; np:hasPublicationInfo sub:pubinfo; a np:Nanopublication . } sub:assertion { nch:Nfkb%20Complex a ProteinComplex: . sub:_1 geneProductOf: hgnc:11929; a Protein: . sub:_2 hasAgent: nch:Nfkb%20Complex; a go:0042789 . sub:_3 occursIn: atcc:CRL-1593.2.aspx, species:9606; rdf:object sub:_2; rdf:predicate belv:increases; rdf:subject sub:_1; a rdf:Statement . sub:assertion rdfs:label "p(HGNC:TNFSF13B) -> tscript(complex(NCH:\"Nfkb Complex\"))" . } sub:provenance { beldoc: dce:description "Approximately 61,000 statements."; dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved."; dce:title "BEL Framework Large Corpus Document"; pav:authoredBy sub:_5; pav:version "1.4" . sub:_4 prov:value "U937 cells were treated with different concentrations of TNFSF13B for 60 minutes at 37 degrees and then assayed for NFKB by EMSA. Also U937 cells were treated with 1 nanomolar of TNFSF13B for various lengths of time to assay the kinetics of NFKB activation all of which indicated that TNFSF13B activates NFKB in a dose and time dependent manner. The gel shift band was specific as its formation could be eliminated with excess unlabeled oligonucleotide and was supershifted by anti-p50 antibody only. To further ascertain NFKB activation was due to proteinaceous nature of TNFSF13B, protein was either digested with trypsin or heat-denatured prior to treatment, both of which completely abolished NFKB activation confirming that TNFSF13B was responsible for this activation."; prov:wasQuotedFrom pubmed:10347144 . sub:_5 rdfs:label "Selventa" . sub:assertion prov:hadPrimarySource pubmed:10347144; prov:wasDerivedFrom beldoc:, sub:_4 . } sub:pubinfo { this: dct:created "2014-07-03T14:29:47.260+02:00"^^xsd:dateTime; pav:createdBy orcid:0000-0001-6818-334X, orcid:0000-0002-1267-0234 . }