http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#Head http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#provenance http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_1 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_2 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0003824 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:104887 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_3 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://amigo.geneontology.org/amigo/term/GO:0008219 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_1 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.w3.org/2000/01/rdf-schema#label cat(p(MGI:Gpx1)) -| bp(GO:"cell death") http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_4 http://www.w3.org/ns/prov#value Selenium-dependent glutathione peroxidase-1 (GPX1) protects against reactive-oxygen-species (ROS)-induced oxidative stress in vivo, but its role in coping with reactive nitrogen species (RNS) is unclear. Our objective was to compare the protection of GPX1 against cytotoxicity of superoxide generator diquat (DQ), NO donor S-nitroso-N-acetyl-penicillamine (SNAP) and peroxynitrite generator 3-morpholinosydnonimine (SIN-1). Primary hepatocytes were isolated from GPX1-knockout (KO) and wild-type (WT) mice and cultured in complete Williams's medium E with various levels of these agents alone or in combination for up to 12 h. While the KO cells were more susceptible to cell death, DNA fragmentation and protein carbonyl formation induced by 0.25-1 mM DQ, these cells were as tolerant as the WT cells to cytotoxicity of 0.1-1 mM SNAP or 0.5-2 mM SIN-1. Treating cells with SNAP (0.1 or 0.25 mM) in addition to DQ produced synergistic cytotoxicity that minimized differences in apoptotic cell death and oxidative injuries between the KO and WT cells. Less protein nitrotyrosine was induced by 0.05-0.5 mM DQ+0.25 mM SNAP in the KO than in the WT cells. Total GPX activity in the WT cells was reduced by 65 and 25% by 0.5 mM DQ+0.1 mM SNAP and 0.5 mM DQ, respectively. Decreases in Cu,Zn-superoxide dismutase (SOD) activity and increases in Mn-SOD activity in response to DQ or DQ+SNAP were greater in the KO cells than in the WT cells. In conclusion, GPX1 was more effective in protecting hepatocytes against oxidative injuries mediated by ROS alone than by ROS and RNS together. Knockout of GPX1 did not enhance cell susceptibility to RNS-associated cytotoxicity. Instead, it attenuated protein nitration induced by DQ+SNAP. http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11672444 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11672444 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#_4 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://purl.org/dc/terms/created 2014-07-03T14:29:58.762+02:00 http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAgXUHytubIo4z11nFMD3oMUmSI1bFr1BTMbvZV6l6fHU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234