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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAgndFMrYFnCgOdQ9xjISp0cneMf3XAc-sodWTF4Iab-0#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAgndFMrYFnCgOdQ9xjISp0cneMf3XAc-sodWTF4Iab-0#_3 http://www.w3.org/ns/prov#value Transcriptional regulation of AE2 {SLC4A2} was also evaluated in differentiated HBE cells stimulated with H2O2. AE2 mRNA expression was analyzed by RT-PCR. A single band corresponding to the 411-base pair product of the AE2 primers was obtained. The relative quantity of the AE2 mRNA was normalized against that of GAPDH (Fig. 5). Minimal AE2 mRNA expression was seen at baseline in the unstimulated cells, despite amplification to 36 cycles. This expression significantly increased within 4 h of exposure to H2O2. 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