http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#Head http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#provenance http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#pubinfo http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:102709 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D008264 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_1 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=9150 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:simvastatin) -> r(MGI:Cav1) http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_3 http://purl.org/dc/elements/1.1/identifier AHA Abstracts P316 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_3 http://purl.org/dc/elements/1.1/title AHA Abstracts P316 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_3 http://purl.org/dc/elements/1.1/type Other http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_4 http://www.w3.org/ns/prov#value Cell death by apoptosis is recognized as a significant event influencing the development and stability of the atherosclerotic lesion. Evidence suggests that the predominant cell type undergoing apoptosis in formed lesions is the macrophage. HMG-CoA reductase inhibitors (statins) have been previously reported to induce apoptosis in several cell types. Their extensive use in the treatment of hypercholesterolemia therefore justifies the examination of the effects of statins on macrophages. Incubation of thioglycollate-elicited mouse peritoneal macrophages (Tg-MPM) with simvastatin (10 ?M) leads to an induction of apoptosis, as measured by DNA fragmentation, cell morphology changes and phosphatidylserine externalization. Simvastatin- induced apoptosis is accompanied by specific induction of caveolin-1 expression, as assessed by immunoblotting. Simvastatin exposure has no significant effect on caveolin-2 expression. The statin- induced caveolin-1 expression is dose- and time- dependent, with a 20- fold induction in macrophages treated with 10 ?M simvastatin for 72 hrs. Increased caveolin-1 expression is at least partially the result of increased caveolin-1 mRNA levels, as simvastatin treatment increases caveolin-1 mRNA 2- fold. Addition of mevalonate (100 ?M) completely prevents the simvastatin- induced apoptosis as well as increase in caveolin-1. Because the simvastatin- mediated increase in caveolin-1 expression correlates with the degree of apoptosis, we also examined caveolin expression in macrophages deprived of glucose or treated with ethanol. Both treatments induced macrophage apoptosis in a time dependent manner with a concomitant and specific increase in caveolin-1 expression. In conclusion, our data suggest that increased caveolin-1 expression in macrophages undergoing apoptosis is not specific to simvastatin action but rather to induction of apoptosis. This suggests that caveolin and cholesterol-rich membrane domains such as caveolae/lipid rafts may be of general importance in the activation of the apoptotic cascade and influence the extent of macrophage apoptosis in the lesion area. http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_3 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_3 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#_4 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM#pubinfo http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://purl.org/dc/terms/created 2014-07-03T14:29:45.373+02:00 http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAhp0dreXOQ8Nfh14iOhWmTrwkmTHkUsrifODQRIOx7RM http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234