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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#_4 http://www.w3.org/ns/prov#value Bacterially expressed heterotrimeric (alpha1, beta1, and gamma1) wild-type, catalytically inactive, and constitutively active forms of AMP-activated protein kinase (AMPK) were used to study phosphorylation by an upstream AMPK kinase preparation. Here, we report the identification of two new phosphorylation sites in the alpha-subunit, viz. Thr258 and Ser485 (Ser491 in the alpha2-subunit) by mass spectrometry, in addition to the previously characterized Thr172 site. Also, autophosphorylation sites in the beta1-subunit were identified as Ser96, Ser101, and Ser108. Mutagenesis of Thr172, Thr258, and Ser485 to acidic residues to mimic phosphorylation in the recombinant proteins indicated that Thr172 was involved in AMPK activation, whereas Thr258 and Ser485 were not. http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12764152 http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12764152 http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#_4 http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w#pubinfo http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w http://purl.org/dc/terms/created 2014-07-03T14:30:12.649+02:00 http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAiurxG6EaFgKA_cYCkjTh70L2cFRGbAnD2ywzgtZs12w http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234