sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_5 ;
    pav:version "1.4" .
  sub:_4 prov:value "Bay 11-7082, an inhibitor of I{kappa}B phosphorylation, and NF-{kappa}B activation was used to determine whether chemokine induction by PGN is NF-{kappa}B dependent. Concentrations of Bay 11-7082 between 1.0 and 5 µM were used according to published studies. RA synovial fibroblasts were pretreated with Bay 11-7082 for 30 min, washed and subsequently stimulated with PGN for 4 h. Total RNA was then extracted and RANTES, GCP-2, and MCP-2 mRNA quantified by real-time PCR. There was a dose-dependent decrease of mRNA levels of the tested chemokines ranging from marked reduction upon treatment with 1 µM Bay 11-7082 to almost complete inhibition in the RA synovial fibroblasts pretreated with 5 µM Bay 11-7082. " ;
    prov:wasQuotedFrom pubmed:14707104 .
  sub:_5 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:14707104 ;
    prov:wasDerivedFrom beldoc: , sub:_4 .
}