. . . . . . . . . . . . . . . "tscript(complex(NCH:\"Nfkb Complex\")) -> r(HGNC:CCL8)" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "1.4" . "Bay 11-7082, an inhibitor of I{kappa}B phosphorylation, and NF-{kappa}B activation was used to determine whether chemokine induction by PGN is NF-{kappa}B dependent. Concentrations of Bay 11-7082 between 1.0 and 5 µM were used according to published studies. RA synovial fibroblasts were pretreated with Bay 11-7082 for 30 min, washed and subsequently stimulated with PGN for 4 h. Total RNA was then extracted and RANTES, GCP-2, and MCP-2 mRNA quantified by real-time PCR. There was a dose-dependent decrease of mRNA levels of the tested chemokines ranging from marked reduction upon treatment with 1 µM Bay 11-7082 to almost complete inhibition in the RA synovial fibroblasts pretreated with 5 µM Bay 11-7082. " . . "Selventa" . . . . "2014-07-03T14:30:19.471+02:00"^^ . . .