http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#Head http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#provenance http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#pubinfo http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://purl.bioontology.org/ontology/MSH/D017209 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0010224 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GO:"response to UV-B") -> bp(MESHPP:Apoptosis) http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_3 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_2 http://www.w3.org/ns/prov#value Ultraviolet radiation is a potent inducer of apoptosis, whereas autocrine nerve growth factor protects human keratinocytes from programmed cell death. To evaluate the role of nerve growth factor in the mechanisms of ultraviolet B-induced apoptosis, cultured human keratinocytes were ultraviolet B irradiated following pretreatment with K252, a specific inhibitor of the tyrosine kinase high-affinity nerve growth factor receptor. Here we report that the addition of K252 significantly enhanced keratinocyte apoptosis. We then transfected normal human keratinocytes with pNUT-hNGF. Nerve growth factor overexpressing keratinocytes secreted the highest amounts of nerve growth factor in culture supernatants, were more viable, and had a higher rate of proliferation than mock-transfected cells. Whereas ultraviolet B radiation downregulated nerve growth factor mRNA and protein as well as the tyrosine kinase high-affinity nerve growth factor receptor in normal keratinocytes, it failed to do so in nerve growth factor-transfected cells. Moreover, nerve growth factor overexpressing keratinocytes were partially resistant to apoptosis induced by increasing doses of ultraviolet B at 24 and 48 h. These results indicate that downregulation of nerve growth factor function plays an important part in the mechanisms of ultraviolet B-induced apoptosis in human keratinocytes. In addition, ultraviolet B caused a decrease in BCL-2 and BCL-xL expression in mock-transfected keratinocytes, but not in nerve growth factor overexpressing cells. Finally, nerve growth factor prevented the cleavage of the enzyme poly(ADP-ribose) polymerase induced in human keratinocytes by ultraviolet B. These results are consistent with a model whereby the autocrine nerve growth factor protects human keratinocytes from ultraviolet B-induced apoptosis by maintaining constant levels of BCL-2 and BCL-xL, which in turn might block caspase activation. http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_2 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10594731 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_3 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10594731 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#_2 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A#pubinfo http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://purl.org/dc/terms/created 2014-07-03T14:29:49.473+02:00 http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAkAR3zQmShu5ppetKhwdj_Qf36_t_q755c46dJuFKd3A http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234