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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAkE4GaXvqEOr2FP3ThlHaTkNXT8gv1PHZ7jH1_Nge3sE#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAkE4GaXvqEOr2FP3ThlHaTkNXT8gv1PHZ7jH1_Nge3sE#_5 http://www.w3.org/ns/prov#value To investigate the normal intracellular distribution of 14-3-3 proteins and their ligands, U2OS cells were immunostained with a polyclonal antibody that recognizes all 14-3-3 isotypes (pan-14-3-3) or with a monoclonal antibody generated against the 14-3-3sigma isotype (Fig. 1 A). Upon growth factor stimulation, Akt is activated, directly phosphorylating FKHRL1 at T32 and S253, and to a lesser extent at S315, and resulting in FKHRL1 relocalization from the nucleus to the cytoplasm .... Similar AKT-dependent changes in subcellular localization have also been observed for the additional FKHRL1 human homologs AFX and FKHR.... 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